Vascular Endothelial Growth Factor 165 (VEGF 165) is integral to angiogenesis, impacting both tumor growth and wound healing. Human VEGF 165 ELISA kits facilitate precise measurement across biological samples, essential for research in cancer and cardiovascular health. Understanding the methodology, sensitivity, and proper handling of these kits is vital for advancing therapeutic strategies.
Understanding VEGF 165 and Its Role in Health
Vascular Endothelial Growth Factor 165, commonly referred to as VEGF 165, plays a crucial role in angiogenesis, the process by which new blood vessels form from pre-existing ones. This protein is vital in both physiological and pathophysiological processes, such as tumor growth and wound healing. VEGF 165, a significant isoform of vascular endothelial growth factor, is essential for supporting endothelial cell proliferation and survival, influencing the overall blood vessel function & behavior of blood vessels.
VEGF 165 ELISA Kits and Sample Types
The Human VEGF 165 ELISA Kit is widely used for the quantitative measurement of this protein in various biological fluids. The kit is designed to work with a variety of sample types, including serum, plasma, and cell culture media with high precision. This versatility makes it an ideal tool for researchers focused on understanding the biological roles of VEGF 165 across different health conditions. Whether analyzing recombinant or natural forms, this kit offers comprehensive support for studying diseases like cancer and diabetic retinopathy.
ELISA Kit Methodology and Sensitivity
The VEGF 165 ELISA kits operate on the sandwich-assay principle, allowing for high specificity and sensitivity in measurement. These kits typically offer a sensitivity as low as 18.75 pg/mL and cover a dynamic range of 31.25 to 2000 pg/mL. This high sensitivity ensures accurate quantification of even low concentrations of VEGF 165 in samples, which is pivotal for detailed research applications like cancer and cardiovascular health studies.
Factors to Consider When Using VEGF 165 ELISA Kits
When selecting sample types for VEGF 165 analysis, researchers must be cautious about the potential cross-reactivity with analogues that could affect results. The kits have demonstrated not only high specificity for VEGF 165 but also excellent recovery rates, indicating their efficacy in research. Various studies have highlighted that achieving consistent, accurate measurements requires careful attention to the kit’s procedural details, including incubation times and temperatures.
Storage and Handling of VEGF 165 ELISA Kit Components
The integrity and performance of an ELISA kit greatly depend on proper storage and handling of its components. Generally, the kits require specific conditions to maintain their effectiveness—most components need storage at 2-8°C. Critical components in these kits, including biotinylated antibodies and HRP conjugates, are sensitive to temperature variations, requiring adherence to guidelines to prevent degradation.
Applications and Research Use of VEGF 165 ELISA Kits
The VEGF 165 ELISA kits are geared toward advanced research applications, providing insights into cellular response mechanisms involved in angiogenesis. They are not intended for clinical diagnostics but rather for expansive studies covering areas from signal transduction to developmental biology. Researchers find these kits beneficial for assessing VEGF 165 levels, particularly where analyzing angiogenic factors may reveal potential biomarkers for therapeutic interventions. In turn, such studies can influence therapeutic strategies in places like oncology and cardiovascular research.
Why You Should Learn More About VEGF 165 ELISA Kits Today
The VEGF 165 ELISA kits are an indispensable tool for researchers determined to understand the intricate processes governing angiogenesis and its impact on various health conditions. By providing precise measurements of VEGF 165 across a range of biological samples, these kits enable significant advancements in research fields like oncology and cardiovascular health. Continuing to explore and understand the role of VEGF 165 can unlock new potential for therapeutic development and provide new insights into disease management and treatment options.
Sources
Abcam Highlights VEGF-165 ELISA Kit Specifications
MSesupplies on VEGF165 Quantitative Determination
LSBio on VEGF 165 Sandwich ELISA Kit