Novogene’s amplicon sequencing is revolutionizing the exploration of microbiomes by focusing on genetic regions like 16S, 18S, and ITS, revealing microbial diversity crucial for ecology and pathogen studies. Delve into the intricate sequencing process, applications, and its robust bioinformatics capabilities that provide detailed insights into microbial communities and ecological interactions.
Understanding Novogene Amplicon Sequencing
Novogene’s amplicon sequencing services are at the forefront of exploring microbiomes in diverse biological and environmental samples. This advanced DNA sequencing method focuses on amplifying specific genetic regions known as 16S, 18S, and ITS, providing a detailed view into microbial diversity and phylogeny of microorganisms. With high-throughput next-generation sequencing, Novogene allows researchers to identify multiple microbial species, offering insights vital for fields like population ecology and disease pathogen identification.
How Does Amplicon Sequencing Work?
The sequencing process begins with sample preparation, where the target DNA regions are isolated and amplified using polymerase chain reactions (PCR). This step is critical for analyzing microbial community structures in complex samples, ensuring that key genes are thoroughly represented throughout the sequencing process. The workflow continues with quality checks, library preparation, sequencing via Illumina platforms, and comprehensive bioinformatics analysis, providing high-quality results suitable for scholarly publication.
Applications and Benefits of Novogene’s Sequencing
Among the many applications of Novogene’s sequencing services is the ability to evaluate microbial communities in animal, plant, and environmental samples. This versatile technique is used to distinguish microbial species and diversity across different ecosystems and geographic regions for both academic and applied research. Amplicon metagenomic sequencing simplifies and expedites the research process compared to whole-genome sequencing, allowing researchers to screen variants effectively, even in GC-rich genomic areas.
Data Analysis and Insights
Through its detailed pipelines, Novogene offers extensive bioinformatics packages, including tools like Qiime2, for detecting single nucleotide changes and creating precise taxa annotations within microbial communities. Analysis outputs, such as heatmaps, Venn diagrams, and Evolutionary Trees, offer a robust visualization of microbial community dynamics, aiding in the understanding of complex ecological interactions and phylogenetic relationships.
Sequencing Methods: Amplicon vs. Shotgun Metagenomic
While amplicon sequencing targets specific genomic areas for detailed taxonomic studies, shotgun metagenomic sequencing offers a more comprehensive view of the entire microbial genome without the need for cultivation. This method is particularly advantageous given that nearly 99% of microorganisms are not cultivable in laboratory settings. Shotgun sequencing provides extensive data, including gene predictions and microbial interactions, critical for in-depth ecological and functional studies.
Why You Should Learn More About Novogene’s Sequencing Services Today
Novogene’s sequencing services provide unparalleled tools for advancing microbial research, offering clear pathways for understanding genetic diversity and ecological interactions. Whether through amplicon sequencing’s specificity or shotgun metagenomic sequencing’s broad scope, researchers gain actionable insights that drive scientific discoveries forward. With proven efficacy, as demonstrated by numerous published studies, Novogene stands out as a leader in genomic services, supporting diverse research needs with its comprehensive analyses and advanced sequencing platforms.
Sources
A Beginner’s Guide to Microbial Sequencing